FastQCFastQC Report
Fri 10 Feb 2017
SRR1614029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1614029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19354595
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC74450.020.7426264
GTAGGAC252600.018.3496173
TGTAGGA279000.017.2362542
TAGGACG306750.016.910274
GTCCTAA170650.016.6724821
AGGACGT306650.016.5929665
GGACGTG305700.016.522586
CTGTAGG282250.016.519811
GTCCTAC212950.016.4399531
TTCGTCA133850.016.38755244
TTAGGAC186100.016.0054573
GACGTGA166500.015.9346847
TTCTCGC134350.015.6742142
TCTCGCC135800.015.44199543
AAGGCGT19000.015.3992466
AGGACCT203350.015.1884775
GACGTGG166550.015.0316977
GGACCTG195700.014.98444756
TTTCTCG144000.014.9641
GCGCCAC14650.014.86731813
Produced by FastQC (version 0.11.5)