FastQCFastQC Report
Wed 3 Aug 2016
DRR028652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136460899
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG166900.027.2201481
ATCGGGA224550.020.1613832
GATCGGA557950.08.924051
AAGCGTC167200.08.7972399
CGGGAGA406600.07.9545434
ATCGGAA658750.07.5332832
TCGGGAG438700.07.4917133
TCGGAAA339150.07.38036443
TCGGGAA296750.07.26133163
GATCCGA123450.06.1825121
AAAGCGT289950.06.02115548
AAGCGGC172450.05.92437559
GCCGGAT132500.05.843612745-49
GAGCGGC212300.05.49493469
TCGGGGG416550.05.14667435-39
Produced by FastQC (version 0.11.5)