FastQCFastQC Report
Tue 2 Aug 2016
DRR028652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136460899
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1884640.13810842620932756TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA597250.055.141861
ATCGGAA648600.050.813272
TCGGAAG726800.045.4135483
CGGAAGA761750.043.2822844
GAGCACA1901250.017.7571499
GAAGAGC1944900.017.4672096
AGAGCAC2098250.016.1210358
TCGTATG409100.016.09265940-44
CGTATGC419100.015.65680140-44
CTCGTAT392350.015.54946640-44
ATGCCGT423150.015.43906645-49
TCTCGTA395950.015.11156940-44
TGCCGTC461850.014.39331745-49
TATGCCG473750.013.97058145-49
GCCGTCT478050.013.79943545-49
CCGTCTT486550.013.34368450-54
AATCTCG460050.013.31029835-39
CGTCTTC527000.012.46251350-54
ATCTCGT502900.011.9958440-44
CGTCTGA569250.011.7446915-19
Produced by FastQC (version 0.11.5)