FastQCFastQC Report
Sun 4 Sep 2016
DRR028650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135258121
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA453550.027.698121
ATCGGAA498900.025.216312
TCGGAAG579800.021.9472453
CGGAAGA623000.020.4602554
TCGTATG273400.09.701529540-44
CGTATGC276950.09.43063740-44
CTCGTAT264800.09.23936540-44
ATGCCGT286250.09.17144645-49
TCTCGTA263550.09.04120740-44
TGCCGTC313200.08.61832345-49
GCCGTCT326700.08.24444545-49
GAGCACA1752550.07.88391459
TATGCCG342600.07.764500645-49
AATCTCG328750.07.54578535-39
GAAGAGC1816350.07.53921136
CCGTCTT353100.07.477711750-54
AGAGCAC1952800.07.03095778
CGTCTTC397800.06.83428350-54
ATCTCGT371400.06.61478140-44
CGTCTGA422800.06.296324715-19
Produced by FastQC (version 0.11.5)