FastQCFastQC Report
Wed 24 Aug 2016
DRR028647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146362980
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG156150.08.3624091
TCCGATC134800.06.64430730-34
ATCGGGA214350.06.49744372
CCGATCG35650.05.691406730-34
CCGATCT172050.05.306852330-34
TGGGCGG421550.05.1798631
AGCGTCG425550.05.170394145
TGGTCGG168250.05.1308911
Produced by FastQC (version 0.11.5)