FastQCFastQC Report
Wed 24 Aug 2016
DRR028647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146362980
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA593700.017.5989511
ATCGGAA641650.016.5565722
TCGGAAG729850.014.7033253
CGGAAGA763600.014.0344814
TCGTATG270050.08.10988740-44
CGTATGC278050.07.860913340-44
CTCGTAT260350.07.755111740-44
TCTCGTA257200.07.664123540-44
ATGCCGT287800.07.5568945-49
TGCCGTC320400.06.941854545-49
GCCGTCT331500.06.656926645-49
TATGCCG342400.06.584749745-49
CCGTCTT357550.06.32135450-54
AATCTCG336600.06.035547335-39
GAGCACA2002000.05.99260859
GAAGAGC2062200.05.8423266
CGTCTTC409150.05.59854750-54
AGAGCAC2179750.05.48732668
ATCTCGT386700.05.371141440-44
Produced by FastQC (version 0.11.5)