FastQCFastQC Report
Thu 14 Jul 2016
DRR028646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151425623
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG165250.07.6044871
TCCGATC135950.06.70591130-34
ATCGGGA220250.05.6305522
CCGATCG35200.05.55875430-34
CGTCGTG404200.05.477861145
CCGATCT170500.05.37253330-34
AGCGTCG428500.05.013733145
Produced by FastQC (version 0.11.5)