FastQCFastQC Report
Thu 14 Jul 2016
DRR028646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151425623
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA604750.016.2179091
ATCGGAA652600.015.2992062
TCGGAAG741150.013.5285343
CGGAAGA777250.012.9375284
TCGTATG280550.07.449342340-44
CGTATGC282400.07.349220340-44
ATGCCGT300800.06.862531745-49
CTCGTAT275150.06.858109540-44
TCTCGTA275750.06.66974240-44
TGCCGTC332650.06.34927645-49
TATGCCG349300.06.100577445-49
GCCGTCT347700.06.06194445-49
CCGTCTT370650.05.780782750-54
GAGCACA2049250.05.61544479
AATCTCG340650.05.444905335-39
CGTCTTC412150.05.304228350-54
GAAGAGC2109600.05.26270346
AGAGCAC2264100.05.06657848