FastQCFastQC Report
Wed 31 Aug 2016
DRR028644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148514877
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA539800.011.5138041
ATCGGAA586550.010.6024262
TCGGAAG683150.09.4105013
CGGAAGA724850.08.9090744
TCGTATG244000.05.498289640-44
CGTATGC249700.05.36697640-44
TCTCGTA236450.05.17754440-44
ATGCCGT260500.05.138833545-49
CTCGTAT238800.05.065922740-44
Produced by FastQC (version 0.11.5)