FastQCFastQC Report
Fri 22 Jul 2016
DRR028638_2.fastq.bz2

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028638_2.fastq.bz2
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142633588
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG148400.017.9976081
ATCGGGA205250.013.104222
GATCGGA446750.08.5939331
ATCGGAA527000.07.3322472
CGGGAGA396400.05.86738444
GCCGGAT127800.05.772534445-49
TCGGAAA316450.05.7701713
AAGCGTC166600.05.30577339
CGCCGGA74500.05.03875345-49
TCGGGAG438800.05.00344663
Produced by FastQC (version 0.11.5)