FastQCFastQC Report
Fri 22 Jul 2016
DRR028638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142633588
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA402800.039.486211
ATCGGAA453650.035.2321622
TCGGAAG540950.029.6412473
CGGAAGA586650.027.2828144
TCGTATG287650.011.39013640-44
CGTATGC291250.011.18961740-44
TCTCGTA272550.010.81374340-44
CTCGTAT274600.010.80164640-44
ATGCCGT307800.010.559175545-49
TGCCGTC341050.09.79751445-49
GAGCACA1810300.09.6026269
GAAGAGC1842000.09.3902026
GCCGTCT358950.09.24431745-49
TATGCCG359200.09.22577745-49
AATCTCG337950.08.929435-39
CCGTCTT375400.08.79709250-54
AGAGCAC2012800.08.6365428
CGTCTGA410800.08.02654715-19
CGTCTTC421500.07.965639650-54
ATCTCGT388600.07.8902840-44
Produced by FastQC (version 0.11.5)