FastQCFastQC Report
Thu 28 Jul 2016
DRR028632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146145208
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA384550.017.6636961
ATCGGAA438600.015.6362312
TCGGAAG522300.013.3109233
CGGAAGA569000.012.29488854
TCGTATG226650.06.28872540-44
CGTATGC233200.06.18670440-44
TCTCGTA225500.05.941419640-44
CTCGTAT227100.05.886790840-44
ATGCCGT250650.05.755857545-49
TGCCGTC287250.05.19409645-49
GCCGTCT300650.05.020467845-49
Produced by FastQC (version 0.11.5)